Podrobnosti o izdelku
Poglej vseISBN
9781588298843Mladinska knjiga ID
399506Leto izida
2009Datum izida
28.02.2009Velikost (šxdxv)
150 × 200 × 10Status dobavljivosti
Na zalogi pri dobaviteljuJezik
ANGZaložnik
SPRINGER-VERLAG GmbHAvtor
CARROLLOpis
Methods and ApplicationsCovers techniques such as cRNA expression in Xenopus oocytes, and examines technologies. This book includes antisense morpholino oligonucleotides, RNAi for knockdown experiments, and the use of integrase to produce transgenic animals, all through microinjection techniques that can be adopted by labs.1. Expression of Exogenous mRNA in Xenopus laevis Embryos for the Study of Cell Cycle Regulation Jill C. Sible and Brian N. Wroble 2. Use of Luciferase Chimaera to Monitor PLC Expression in Mouse Eggs Karl Swann, Karen Campbell, Yuansong Yu, Christopher Saunders, and F. Anthony Lai 3. Analysis of 14-3-3 Family Member Function in Xenopus Embryos by Microinjection of Antisense Morpholino Oligos Jeffrey M. C. Lau and Anthony J. Muslin 4. A Microinjectable Biological System, the Xenopus Oocyte, as an Approach to Understanding Signal Transduction Protein Function Katia Cailliau and Edith Browaeys-Poly 5. Combining Microinjection and Immunoblotting to Analyze MAP Kinase Phosphorylation in Single Starfish Oocytes and Eggs David J. Carroll and Wei Hua 6. Analysis of Signaling Pathways in Zebrafish Development by Microinjection William H. Kinsey 7. Protein Inhibition by Microinjection and RNA-Mediated Interference in Tissue Culture Cells: Complimentary Approaches to Study Protein Function Jane R. Stout, Rania S. Rizk, and Claire E. Walczak 8. DNA Delivery by Microinjection for the Generation of Recombinant Mammalian Cell Lines Sebastien Chenuet, Madiha Derouazi, David Hacker, and Florian Wurm 9. Bacteriophage fC31 Integrase Mediated Transgenesis in Xenopus laevis for Protein Expression at Endogenous Levels Bryan G. Allen and Daniel L. Weeks 10. Germline Transformation of Caenorhabditis elegans by Injection Pavan Kadandale, Indrani Chatterjee, and Andrew Singson 11. Quantitative Microinjection of Mouse Oocytes and Eggs Douglas Kline 12. Microinjection of Follicle-Enclosed Mouse Oocytes Laurinda A. Jaffe, Rachael P. Norris, Marina Freudzon, William J. Ratzan, and Lisa M. Mehlmann 13. Functional Studies of Regulatory Genes in the Sea Urchin Embryo Vincenzo Cavalieri, Maria Di Bernardo, and Giovanni Spinelli 14. Exploring the Cytoskeleton during Intracytoplasmic Sperm Injection in Humans Vanesa Y. Rawe and Hector Chemes 15. Somatic Cell Nuclear Transfer in the Mouse Satoshi Kishigami and Teruhiko Wakayama
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